SPL Cell Culture Flask 75 cm2 T75 Filter Cap Non‑Treated Sterile

Description

Product Overview

The SPL Cell Culture Flask 75 cm2 (T75) is engineered for high‑performance cell culture applications in research and development laboratories. Constructed from high‑quality, non‑treated glass, the flask provides a chemically inert surface that promotes uniform cell attachment and proliferation. The integrated white filter cap incorporates a 0.2 µm membrane, allowing sterile filtration of gases while preventing contamination during media changes. The short, wide, angled neck design facilitates effortless pipetting and reduces the risk of spillage, making routine handling more efficient. With a growth area of 75 cm², the flask accommodates a broad range of cell densities, from low‑density primary cultures to high‑density suspension lines. The ergonomic shape enhances stackability, optimizing incubator space without compromising accessibility.

Usage

This flask is ideal for a variety of cell culture workflows, including adherent mammalian cells, stem cell maintenance, and transient transfection protocols. Its sterile, non‑treated surface is compatible with a wide spectrum of culture media, supplements, and extracellular matrix coatings. Researchers can employ the filter cap to perform gas exchange without exposing the culture to the external environment, thereby preserving the aseptic conditions essential for sensitive cell lines. The flask’s robust construction tolerates repeated autoclave cycles, making it suitable for long‑term studies and high‑throughput screening. Whether used in standard CO₂ incubators, hypoxia chambers, or bioreactor setups, the flask delivers consistent performance across diverse experimental conditions.

Why Choose Us

Choosing the SPL Cell Culture Flask means selecting a product backed by rigorous quality assurance processes. Each flask undergoes comprehensive testing for pyrogenicity, cytotoxicity, and nucleic acid contamination, guaranteeing that it is DNase‑free, RNase‑free, and free of human DNA. This level of purity minimizes background interference in downstream assays such as PCR, ELISA, and western blotting. The manufacturer, SPL Life Sciences, leverages decades of expertise in glassware fabrication to deliver a product that meets international standards for laboratory consumables. In addition, the company provides responsive technical support and a clear return policy, ensuring that any issues are resolved promptly and professionally.

Key Features

• Sterile, non‑treated glass surface promotes optimal cell attachment and growth.
• Integrated white filter cap with 0.2 µm membrane enables safe media exchange without contamination.
• Short, wide, angled neck design provides easy access for pipetting and reduces spillage.
• Stackable ergonomic shape maximizes incubator space while maintaining stability.
• Comprehensive quality testing ensures pyrogen‑free, DNase/RNase‑free, and human DNA‑free performance.
• Durable construction tolerates repeated autoclave cycles for long‑term use.
• Responsive customer support and clear return policy for peace of mind.

FAQ

Can the flask be used for both adherent and suspension cell cultures?

Yes, the flask’s flat bottom and generous growth area support adherent cells, while its sterile environment and filter cap are suitable for suspension cultures that require frequent media changes.

Is the filter cap compatible with standard pipette tips?

The filter cap is designed with a standard 22‑mm opening, allowing direct use of most laboratory pipette tips and syringes without the need for adapters.

How many autoclave cycles can the flask withstand?

The flask is rated for up to 20 autoclave cycles at 121 °C, maintaining its structural integrity and sterility throughout repeated sterilization procedures.

What measures are taken to ensure the flask is free of nucleic acid contamination?

Each batch undergoes stringent DNase and RNase testing, and the manufacturing process includes dedicated clean‑room environments to prevent human DNA contamination.

What is the recommended storage condition for the flasks before use?

Store the flasks in a clean, dry area at room temperature. Keep them sealed in their original packaging until ready for use to maintain sterility.